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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Jan 21;30(4):758–765. doi: 10.1161/ATVBAHA.109.199893

Figure 3. The effect of 8 Br-cGMP on endothelial inflammation and insulin signaling.

Figure 3

HMEC were treated with 50 µM 8-Br-cGMP for 12 h in the presence or absence of ODQ (1 µM) and then stimulated with LPS (5 ng/ml) for 1 h or palmitate (100 µM) for 3 h. A.B. Phospho-IκBα levels were assessed by Western blot and normalized to GAPDH and fold increased over the control condition was calculated following densitometry. C. Following treatment with vehicle or 8-Br-cGMP, HMEC were stimulated with 100 nM insulin for 15 min and pAkt levels were assessed by Western blot. * p<0.05.