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. Author manuscript; available in PMC: 2010 May 6.
Published in final edited form as: Integr Biol (Camb). 2009 Feb 26;1(4):301–310. doi: 10.1039/b900685k

Fig. 2.

Fig. 2

Dynamic force spectrum (DFS) of bilayer hemifusion. With SNAREs in the opposing bilayers, the compression (fusion) force was significantly reduced as compared to SNARE-free bilayers. This revealed facilitation of membrane fusion due to the interaction of VAMP (v-SNARE) with syntaxin/SNAP-25 (v-SNAREs) in the opposite bilayers. Omission of SNAP-25 from the t-SNARE bilayers resulted in partial fusion facilitation due to the direct weaker binary interaction of VAMP with syntaxin. Lines are fits of eqn (1) to the data points. Force values at the different compression rates were derived from distribution histograms of force measurements that were acquired in triplicate experiments on different days (see Materials and methods section). Error bars represent the standard error of the mean (s.e.m.) of all the force values in the distribution histograms of the same compression rates (n; 50 < n < 312).