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. Author manuscript; available in PMC: 2010 May 6.
Published in final edited form as: Integr Biol (Camb). 2009 Feb 26;1(4):301–310. doi: 10.1039/b900685k

Fig. 5.

Fig. 5

SNARE perturbations interfere with the mechanical strength of the SNARE complex. The unbinding force of the SNARE complex is significantly reduced upon cleavage of VAMP 2 in the v-SNARE bilayers with BoNT/B or omission of SNAP-25 from the t-SNARE bilayers. A further reduction in the SNARE binding strength was observed in presence of mut1-SNAP-25 or mut2-SNAP-25 in the t-SNARE bilayers as compared to the absence of SNAP-25. This was interpreted as the result of the interference of truncated SNAP-25 mutants with the direct binary interaction of VAMP with syntaxin; hence, a weaker SNARE interaction takes place in presence of mut1-SNAP-25 or mut2-SNAP-25 as compared to the complete absence of SNAP-25. However, the strongest v-/t-SNARE interaction takes place in presence of the full-length SNAP-25 in the a binary syntaxin/SNAP-25 complex in the t-SNARE bilayers. Error bars are the s.e.m. (16 < n < 139).