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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Cancer Prev Res (Phila). 2010 Apr 27;3(5):640–652. doi: 10.1158/1940-6207.CAPR-09-0170

Figure 3. Effects of NMU and MSC on the plasma melatonin concentrations and MTNR1A mRNA expression levels in mammary gland and liver.

Figure 3

(A) Plasma melatonin levels were measured by ELISA in plasma samples of rats in different groups as described in Fig. 1 legend; (B and C) Relative mRNA expression levels were determined using quantitative real-time RT-PCR with total RNA samples from mammary gland (MG) (B) and liver tissues (C) of rats as described previously; (D) Conservation of Bmal1 E-box elements (framed) in the promoter regions of rat MTNR1A and circadian genes. The E-boxes within the 10-kb of 5’ up-stream, exon 1, and 2-kb of intron 1 region were analyzed. r, rat; m, mouse; h, human (a) Alignment of the proximal MTNR1A promoter regions from rat, mouse, and human. (b) Sequence comparison of the E-boxes found in MTNR1A promoter with those in the promoter regions of the indicated circadian genes in rat.

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