Table I.
Conformational Free-Energy Change per Molal of Solute Activity
| Spin label | Solute | ΔΔG° (kcal/mol)a |
|---|---|---|
| BtuB | ||
| T3R1 | Sucrose | 0.35 ± 0.05 |
| T3R1 | PEG 400 | 0.16 ± 0.03 |
| T3R1 | PEG 1000 | 0.45 ± 0.05 |
| T3R1 | PEG 3350 | 0.61 ± 0.07 |
| T3R1 | PEG 6000 | 0.95 ± 0.1 |
| FecA | ||
| A81R1 | PEG 400 | 0.9 ± 0.2 |
| A81R1 | PEG 1000 | 1.1 ± 0.2 |
| A81R1 | PEG 3350 | 1.4 ± 0.3 |
| A81R1 | PEG 6000 | 1.9 ± 0.5 |
| V85R1 | PEG 400 | 1.0 ± 0.2 |
| V85R1 | PEG 1000 | 1.1 ± 0.2 |
| V85R1 | PEG 3350 | 1.9 ± 0.3 |
| V85R1 | PEG 6000 | 2.0 ± 0.5 |
Errors in the free energies are ∼20% or less and are based on the standard deviations obtained from multiple measurements.