Figure 1.

R26Notch2 mice are generated. (A) Targeting strategy of Flag-Notch2 in the Rosa26 locus. A Flag-Notch2 fragment is inserted into the pBigT vector containing an adenovirus splice acceptor sequence followed by a PGK-neo cassette and a tpA stop sequence flanked by two loxP sites. The resultant plasmid is inserted into pRosa26pl239pA (a modified pRosa26-1 vector) and subsequently integrated into the Rosa26 locus in ES cells by homologous recombination. (B) Targeted ES clones are confirmed by Southern blot analysis. Genomic DNA is digested with EcoRI and hybridized with probe A. (C) PCR amplification of mouse tail DNA.