Figure 3.

HG induces SOCS expression in cultured renal cells. (A) HK2 cells were incubated for different time intervals in culture medium containing HG (30 mM d-glucose, black bars) or mannitol (10 mM d-glucose + 20 mM d-mannitol; gray bars). Gene expression of SOCS1 (upper panel) and SOCS3 (lower panel) was analyzed by real-time PCR and values were normalized by 18S expression. (B) Representative immunoblots and densitometric analysis showing time course of SOCS1, SOCS3, P-JAK2, and P-STAT1 induction by HG in human MCs. Fold increases versus basal conditions (10 mM d-glucose) are mean ± SEM of three to five experiments in duplicate (*P < 0.05 versus basal).