Table 1.

Inhibitory effect of Jeju seaweeds on the production of pro-inflammatory mediators

Specimen number	Scientific name	Inhibition rate (%)					Phlorotannin content (µg/ml)
		NO	PGE2	IL-6	TNF-α	Cytotoxicity
JBR243	Sargassum nipponicum	72.1±2.7**	86.2±0.8**	74.9±7.2**	90.0±2.6**	132.6±5.2	58.0
JBR256	Myelophycus simplex	81.9±0.3**	65.0±2.2**	74.5±4.5**	51.8±1.2**	103.5±1.6	33.0
JBR270	Chondrus ocellatus	78.2±0.3**	90.7±1.0**	83.1±2.4**	71.0±1.9**	106.1±12.4	37.2
JBR271	Ishige okamurae	30.8±0.9*	NE	47.1±0.7*	3.1±0.8*	118.6±3.8	332.5
JBR274	Bonnemaisonia hamifera	81.8±0.1**	NE	18.0±0.6**	73.8±0.0**	99.5±1.0	72.2
JBR345	Scytosiphon lomentaria	70.4±0.2**	89.9±1.5**	90.4±0.2**	55.2±0.3**	93.6±6.9	65.0
JBR354	Callophyllis crispata	83.6±0.0**	84.7±3.1**	90.4±0.5**	75.7±0.9**	79.7±5.9	40.0
JBR368	Padina arborescens	59.7±0.4**	80.5±0.9**	71.3±3.7**	80.9±0.7**	87.8±8.7	124.0
JBR375a	Laurencia okamurae	82.4±0.1**	82.1±4.2**	76.9±0.7**	52.5±0.0**	91.4±2.4	37.3
JBR376	Chondria crassicaulis	75.7±0.0**	88.4±1.6**	84.8±0.6**	67.1±0.3**	87.5±5.3	54.7
JBR510b	Grateloupia elliptica	78.2±0.4**	81.0±4.8**	85.7±0.8**	63.8±0.8**	89.4±6.9	47.7
JBR511b	Sargassum thunbergii	77.3±0.2**	73.8±4.0**	46.9±4.4**	63.1±1.2**	98.0±3.7	44.8
JBR518b	Gloiopeltis furcata	77.6±0.5**	85.6±0.6**	82.1±2.2**	70.9±1.3**	108.3±4.8	53.3
JBR538b	Hizikia fusiformis	77.2±0.1**	81.0±4.8**	69.9±6.7**	61.5±3.9**	105.8±3.5	43.3

The presence of nitrite, as a stable oxidized product of NO, was determined in cell-culture media by a modified Griess method. The amounts of PGE₂, TNF-α, and IL-6 in the cell-culture supernatant and serum were measured using an ELISA kit. RAW 264.7 cells were incubated with various concentrations of seaweed extracts (25^a, 50^b, or 100 µg/ml). Cell viability was determined by MTT assay. The data represent the mean±SD of triplicate experiments

^* P<0.05 vs. LPS alone

^** P<0.01 vs. LPS alone

NE: no effects