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DIC and edge-detected ROIs in a similar experiment using MMP-8. (a) Initial strain on collagen fibrils formed between pipettes. ROIs are located as described previously, where S denotes a strained region and U denotes an unstrained region. The series of four boxes to the right of each large image are represented as described previously. Follow-on images (b,c) show the pattern of degradation as a function of time. These are preliminary unpublished results. Scale bar, 10 µm (a–c).
