Figure 5.

DIC and edge-detected ROIs. (a) Intial strain on a heavily manipulated network of collagen fibrils. ROIs are strained (S) or unstrained (U). The translucent circle demarcates an area where collagen fibrils have been heavily compacted by our attempt to tension fibrils. The series of four boxes to the right of each large image represent: (upper left) high magnification of strained ROI, (upper right) corresponding detected edges, (lower left) high magnification of unstrained ROI and (lower right) corresponding edge-detected images. (b,c) Follow-on images showing the degradation sequence. Scale bar, 20 μm (a–c).