FIGURE 3.
FRH causes histologic evidence of LPS-induced lung injury prior to neutrophil recruitment. H&E stains of paraffin-embedded lungs of animals exposed to LPS 50 µg and euthermia for 24 h, original magnifications ×200 (A) and ×400 (B), or hyperthermia, ×200 (C) and ×400 (D). The animals exposed to LPS and hyperthermia have more airway edema, alveolar septal thickening, and pyknotic nuclei (arrows) in the alveolar walls as compared with LPS alone. After 48 h, the lungs of animals exposed to LPS 50 µg and euthermia, original magnifications ×200 (E) and ×400 (F), or hyperthermia, ×200 (G) and ×400 (H) show that hyperthermia exaggerates delayed neutrophil recruitment.