Figure 4.—
Temporal requirements for the expression of CycY. Embryos from a CycYE8/CyO; hs-CycY/TM3, Ser self-cross were collected for 24 hr and heat shocked for different regimes. Each row indicates a different heat-shock schedule. On the left side, each bar represents a single 1-hr heat shock at 37° on that particular day. The efficiencies of rescue to adulthood are shown on the right. The genotype of each adult was determined by the presence or absence of CyO and Ser balancer chromosomes (materials and methods). Representative adult genotypes were confirmed by single-fly PCR. For each condition, the total number of adults analyzed was between 200 and 300.