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qRT–PCR results for the D. ananassae and D. grimshawi retrogenes (Da_ran-like, Da_Ntf-2r, Dg_ran-like, and Dg_ Ntf-2r) are shown. The average of the retrogene CT difference to the normalizing gene (i.e., ΔCT) and standard errors are shown for every tissue type. f, females; m, males.
