TABLE 1.
Strains used in this study
| Strain | Genotypea | Source or reference |
|---|---|---|
| TR10000 | Wild-type S. enterica (serovar Typhimurium) LT2 | Lab collection |
| TT22240 | recA643∷Tn10dTc | Williams et al. (2006) |
| TT25467 | his-644(ΔOGDCBHAF) pro-621/F′128pro+lacI4650∷Ka(sw, KnS), lacZY+/pSIM5(CmR) | This report |
| TT25468 | his-644(ΔOGDCBHAF) pro-621 recA650∷Gnt(sw)/F′128pro+lacI4650∷Ka(sw, KnS), lacZY+ /pSIM5(CmR) | This report |
| TT25469 | his-644(ΔOGDCBHAF) pro-621 recA651∷Rif(sw)/F′128pro+lacI4650∷Ka(sw, KnS), lacZY+/pSIM5(CmR) | This report |
| TT25478 | pyrD2266∷Tn10(TcSKn)/pSIM5(CmR) | This report |
| TT25479 | pyrD2266∷Tn10(TcSKn) recA650∷GntR(sw)/pSIM5(CmR) | This report |
| TT25486 | argH1823∷Tn10(TcSKn)/pSIM5(CmR) | This report |
| TT25487 | argH1823∷Tn10(TcSKn) recA650∷Gnt(sw)/pSIM5(CmR) | This report |
| TT25706 | argH1823∷Tn10(T-Recs) recA650∷Gnt(sw) | This report |
| TT25707 | pyrD2266:Tn10(T-Recs) recA650∷Gnt(sw) | This report |
| TT25791 | Wild-type LT2/F′128lacI4650∷Ka(sw, KnS), lacZY+ | This report |
| TT25792 | recA651∷Rif(sw)/F′128lacI4650∷Ka(sw, KnS), lacZY+ | This report |
| TT25794 | leuD21 proB1657∷Tn10 recA650∷Gnt(sw)/F′128 pro+ IS3C∷Rif(sw) ΔlacIZ4652∷KnR(sw) lacA4653∷(CmR, OcrecA+) | This report |
| TT25996 | argH1947∷Lac[lacI4650∷Ka(sw,KnS), lacZY+]/pSIM5(CmR) | This report |
| TT25997 | argH1947∷Lac[lacI4650∷Ka(sw,KnS), lacZY+] recA650∷Gnt(sw)/pSIM5 (CmR) | This report |
| TT26002 | pyrD2827∷Lac[lacI4650∷Ka(sw,KnS), lacZY+]/pSIM5 (CmR) | This report |
| TT26003 | pyrD2827∷Lac[lacI4650∷Ka(sw,KnS), lacZY+] recA650∷Gnt(sw)/pSIM5 (CmR) | This report |
| TT26046 | argH1947∷Lac[lacI4650∷Ka(sw,KnS), lacZY+] pyrD2826∷Kan recA650∷Gnt(sw)/pSIM5 (CmR) | This report |
| TT26047 | argH1947∷Lac[lacI4650∷Ka(sw,KnS), lacZY+] purH2378∷Kan recA650∷Gnt(sw)/pSIM5 (CmR) | This report |
| TT26049 | pyrD2827∷Lac[lacI4650∷Ka(sw,KnS), lacZY+] purH2378∷Kan recA650∷Gnt(sw)/pSIM5 (CmR) | This report |
| TT26050 | pyrD2827∷Lac[lacI4650∷Ka(sw,KnS), lacZY+] hisC10309∷Kan recA650∷Gnt(sw)/pSIM5 (CmR) | This report |
| TT26056 | his-644(ΔOGDCBHAF) proAB670∷sw-SpcRrecA650∷Gnt(sw)/F'128pro+ lacI4650∷Ka(sw,KnS), lacZY+] IS3A∷Kan(sw)/pSIM5(CmR) | This report |
| TT26057 | his-644(ΔOGDCBHAF) proAB1657(TcR) recA650∷Gnt(sw)/F′128pro+ lacI4650∷Ka(sw,KnS), lacZY+] IS3A∷Kan(sw)/pSIM5(CmR) | This report |
| TT26059 | his-644(ΔOGDCBHAF) proAB670(SpR) recA650∷Gnt(sw)/F′128pro+ DUP2066[lacI4650∷Ka(sw,KnS), lacZY+]–IS3ACjoin–[ΔlacIZ4652∷Kan(sw)]/pSIM5(CmR) | This report |
| TT26061 | his-644(ΔOGDCBHAF) proAB1657(TcR) recA650∷Gnt(sw)/F′128pro+ DUP2066[lacI4650∷Ka(sw,KnS), lacZY+]–IS3ACjoin–[ΔlacIZ4652∷Kan(sw)]/pSIM5(CmR) | This report |
| TT26065 | his-644(ΔOGDCBHAF) proAB1657(TcR) recA650∷Gnt(sw) /F'128pro+ DUP2067[lacI4650∷Ka(sw,KnS), lacZY+]–REP25/REP32/17 join –[ΔlacIZ4652∷Kan(sw)]/pSIM5(CmR) | This report |
| TT26066 | his-644(ΔOGDCBHAF) proAB670(SpR) recA650∷Gnt(sw)/F′128pro+ DUP2067[lacI4650∷Ka(sw,KnS), lacZY+]–REP25/REP32/17 join –[ΔlacIZ4652∷Kan(sw)]/pSIM5(CmR) | This report |
| TT26069 | his-644(ΔOGDCBHAF) proAB1657(TcR) recA650∷Gnt(sw)/F′128pro+ DUP2068[lacI4650∷Ka(sw,KnS), lacZY+]–REP26/REP32/17 join–[ΔlacIZ4652∷Kan(sw)]/pSIM5(CmR) | This report |
| TT26070 | his-644(ΔOGDCBHAF) proAB670(SpR) recA650∷Gnt(sw)/F′128pro+ DUP2068[lacI4650∷Ka(sw,KnS), lacZY+]–REP26/REP32/17 join—[ΔlacIZ4652∷Kan(sw)]/pSIM5(CmR) | This report |
| TT26073 | his-644(ΔOGDCBHAF) proAB1657(TcR) recA650∷Gnt(sw)/F′128pro+ DUP2069[lacI4650∷Ka(sw,KnS), lacZY+]–IS3BCjoin–[ΔlacIZ4652∷Kan(sw)]/pSIM5(CmR) | This report |
a
“sw” designates alleles in which the normal sequence was replaced by a drug resistance determinant. “T-Recs” designates a derivative of Tn10 with a CmR determinant and a constitutive (Oc) recA+ allele inserted into the middle of the Tn10 tetA gene, rendering the strain sensitive to tetracycline and resistant to chloramphenicol. “lacI4650∷Ka(sw, KnS)” refers to a lacI gene replaced by a promoterless kanamycin resistance determinant. “TcSKn” designates a Tn10dTc element in which a KnR determinant is inserted in the tetA gene, rendering the strain sensitive to tetracycline and resistant to kanamycin. The “pSIM5(CmR)” plasmid carries the recombination genes (red) of phage lambda.