Figure 3.—

Abrogation of recombination suppresses the nbs1Δ slowing defect. (A) The slowing defect displayed by MRN mutants is recombination dependent. The partial slowing defect seen in MRN mutants is suppressed in the nbs1Δ rhp51Δ (yFS639, n = 3), rad32Δ rhp51Δ (yFS704, n = 2), and rad50Δ rhp51Δ (yFS705, n = 1) double mutants. nbs1Δ defects are also suppressed in the nbs1Δ swi5Δ (yFS640, n = 4) and nbs1Δ swi1Δ (yFS709, n = 2) double mutants. (B) Both nbs1Δ (yFS267, data from 1B) and sfr1Δ (yFS201, n = 3) mutants display a partial defect in slowing while the nbs1Δ sfr1Δ (yFS707, n = 1) mutant displays a complete defect. This defect is suppressed in the nbs1Δ sfr1Δ rhp51Δ (yFS708, n = 2) triple mutant.