Table 1.
Effect of Kinesin-8 on microtubule-shortening rate and catastrophe frequency
| Parameter | Experiment type and organism | With Kinesin-8a | Without Kinesin-8b |
|---|---|---|---|
| Microtubule-shortening rate (μm/min) | In vivo: budding yeast astral microtubules | 1.91 ± 0.69 [16] | 2.82 ± 0.95 [16] |
| In vivo, human (HeLa cells) | 1.92 ± 0.06 [15] (metaphase) | 2.80 ± 0.08 [15] (metaphase) | |
| 1.68 ± 0.08 [15] (anaphase) | 2.12 ± 0.14 [15] (anaphase) | ||
| In vitro, GMPCPP microtubules | ~0.10 [16] (50 nM Kip3p) | ~0.00 [16] | |
| ~0.50 [8] (3.3 nM Kip3p) | ~0.00 [8] | ||
| In vitro, GMPCPP microtubules | ~1.25 [9] (100 nM Kif18A) | ~0.00 [9] | |
| Microtubule catastrophe frequency (min−1) | In vivo, budding yeast astral microtubules | 0.48 [16] | 0.27 [16] |
a
Kinesin-8 molecular motors are present in the assay, either endogenously as in the in vivo studies, or added to the assay in the noted concentrations as in the in vitro studies.
b
Kinesin-8 molecular motors are either deleted or knocked down as in the in vivo studies, or absent as in the in vitro studies.