Table 3.

Agreement between the results observed by 2-DE gel analysis and immunoblotting

		2-DE Gels		Immunoblotting
Protein	Spot #	MW (kDa)	Changea	MW (kDa)	Changea	Concordance
Fibronectin	17	218	↓	273	↓	Yes
Fibronectin	23	133	↓	166	↓	Yes
Peroxiredoxin-1	18	23	↓	23	↓	Yes
Tenascin-C	17	218	↓	260	↓	Yes
				200	–	No
			↓	170	↓	Yes
			↓	133	↓	Yes
GAPDH	3	38	↑	36	↓	No
Peroxiredoxin-2	1	22	↑	23	↓	No
Peroxiredoxin-3	16	16	↓	23	↑	No
	20		↓			No
VDAC2	3	38	↑	38	↓	No
Apolipoprotein A1	1	22	↑	30	–	No
Collagen VI	23	133	↓	100	–	No
myosin light chain 2	2	16	↓	20	–	No
Peroxiredoxin-6	18	23	↓	25	–	No

^aDirection of difference in spot and band intensity compared to WT; GAPDH – glyceraldehyde-3-phosphate dehydrogenase; VDAC2 – voltage-dependent anion channel 2