Resolution range (Å) |
8.0 – 1.05 |
8.0 – 0.93 |
No of atoms |
|
|
Amino acid residues |
2,622 |
2,622 |
Sugar residues |
39 |
39 |
Heme |
43 |
43 |
CaII
|
2 |
2 |
MnII
|
- |
1 |
Glycerol |
24 |
12 |
Water |
549 |
477 |
Rwork [working set, Fo > 4σ(Fo)] |
11.1 (144,730) |
10.7 (191,300) |
Rwork (working set, all reflections) |
11.7 (156,881) |
12.4 (243,170) |
Rfree [test set, Fo > 4σ(Fo)] |
13.6 (3,181) |
12.4 (3,967) |
Rfree (test set, all reflections) |
13. 9 (3,352) |
13.4 (4,736) |
Rcryst [working + test set, Fo > 4σ(Fo)] |
11.1 (147,911) |
10.8 (195,267) |
Rcryst (working + test set, all reflections) |
11.6 (160,232) |
12.4 (247,906) |
No. of Parameters |
30,135 |
29,226 |
No. of observations/No. of parameters |
5.3 |
8.5 |
No. of restraints |
36,426 |
35,548 |
Mean isotropic B-factor (Å2) |
|
|
All atoms |
12.973 |
13.670 |
Main chain atoms |
9.279 |
10.098 |
Side chain atoms |
11.776 |
13.276 |
Sugar residues |
17.171 |
16.416 |
Heme and metal ions |
8.394 |
7.150 |
Solvent |
24.909 |
25.687 |
RMS deviation from ideal geometry |
|
|
Bond length (1-2) (Å) |
0.017 |
0.018 |
Angle distance (1-3) (Å) |
0.038 |
0.033 |
Chiral volume (Å3) |
0.129 |
0.096 |
Non-zero chiral volume (Å3) |
0.219 |
0.105 |
Deviation from planes (Å) |
0.029 |
0.028 |