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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: Biochem J. 2009 Aug 27;422(3):433–442. doi: 10.1042/BJ20090014

Figure 1. EphB4 siRNA knock down decreases EphB4 expression and increases cancer cell adhesion to fibronectin.

Figure 1

(A) Lysates from control transfected cells and from cells transfected with 4 individual (1 through 4) or pooled EphB4 siRNAs were probed by immunoblotting for EphB4 72 hours after transfection. Anti-actin or anti-tubulin antibodies were used as protein loading controls. (B) The indicated cell lines were transfected with control or pooled EphB4 siRNAs and allowed to attach to fibronectin for 15 min (FN 1) or 30 min for the MDA-MB-435 cells and 1 hour for the MCF7 cells (FN 2). As a control, cells were allowed to attach to poly-l-lysine (PLL) for 1 hour. The histogram shows average numbers of attached cells ± SEM from 3 coverslips. **P<0.01 or ***P<0.001 compared to control siRNA using one-way ANOVA and Bonferroni’s post-hoc test. (C) siRNA transfected cells were triple labeled to visualize focal adhesions (green: paxillin or phosphotyrosine), the actin cytoskeleton (red: rhodamine phalloidin) and nuclei (blue: DAPI). The scale bar represents 10 μM.