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. Author manuscript; available in PMC: 2011 Mar 24.
Published in final edited form as: J Am Chem Soc. 2010 Mar 24;132(11):3914–3922. doi: 10.1021/ja910578y

Figure 1.

Figure 1

ONOO--induced structural perturbations in COX-1 are driven by heme. (a) Absorbance spectra were acquired for the reconstitution of apoCOX-1 (0.21 mg/ml) at 25°C with Fe+3PPIX (heme; FePP) and (b) Co+3PPIX (CoPP) from stock solutions (500 μM each). (c) Circular dichroism (CD) spectra acquired in the far UV region at 25°C compare the structure of apoCOX-1 (dashed) to FePPCOX-1 (solid black). The red CD spectrum was acquired following incubation of FePPCOX-1 with ONOO- (250 μM). (d) CD spectra were acquired as in (c), but for COX-1 reconstituted with CoPP.