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ONOO- is a stimulus for heme-dependent COX-1 multimer formation. Western blots for the reaction of reconstituted COX-1 (0.12 mg/ml) with ONOO- (250 μM) in the presence (+) and absence (-) of (a) arachidonic acid (AA; 100 μM), eicosapentanoic acid (EPA; 100 μM), aspirin (70 μM), and indomethacin (14 μM) and (b) AA (100 μM) and glutathione (GSH; 0.25 mM) in combination with glutathione peroxidase (GSH-Px: 25 U/ml). Blots were probed with monoclonal anti-nitrotyrosine (anti-3-NT).
