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. Author manuscript; available in PMC: 2010 May 10.
Published in final edited form as: Exp Eye Res. 2008 Jul 12;87(4):376–384. doi: 10.1016/j.exer.2008.07.002

Figure 2. Partial mapping of myocilin monoclonal antibody epitopes.

Figure 2

(A) Myocilin is cleaved at amino acid 214 in conditioned media isolated from 293T cells overexpressing myocilin. Antibodies 1.1, 7.1, and 11.1 recognize full-length myocilin (amino acids 33–504). Antibodies 5.1, 12.1, and 20.1 recognize full-length myocilin and a 35 kDa C-terminal fragment (amino acids 215–504). (B) Identification of myocilin mutant Gln368Stop by C-terminal antibodies 5.1 and 20.1 suggests the epitope for those two myocilin monoclonal antibodies is found between amino acids 215–368. The inability of myocilin monoclonal antibody 12.1 to recognize Gln368Stop suggests its epitope is found between amino acids 369 and 504. Arrows indicate full-length myocilin. Asterisks indicate Gln368Stop product. Lowercase C indicates C-terminal fragment. Polyclonal antibodies Myoc-N (N-terminus) and Myoc-C (C-terminus) served as controls.