. Author manuscript; available in PMC: 2011 Apr 6.

Published in final edited form as: Biochemistry. 2010 Apr 6;49(13):2925–2931. doi: 10.1021/bi9021439

Table I.

Sense primers for generation of human PCFT mutants used in the current study

Mutations	Primer sequence (5′ to 3′)
C21S	GCGGCTGCCGTGCTGTCCCGGGGCCCGGTAGAG
C66S	CGCCAAAGGGGGGGCTCCAGCAACCGCAGCGCG
C151S	CTGGGTCGCATCCTTTCTGCCCTCCTCGGCGAC
C229S	CTCTATGCAGCTTTCTCCTTTGGTGAGACCTTA
C298S	CTAAGCACACCCCTCTCCTGGGACTCCAAACTA
C328S	AAGCTCCTGCAGTACTCCCTGGCCGATGCCTGG
C397S	TTTTCTGCTGTGGCCTCTGTGAAATAGCCTGGCC
S110C	GGAGC TTGGAGCGAC TGTGTGGGCCGCCGCCCG
V141C	CAGCTGCAGCTCCACTGCGGCTACTTCGTGCTG
S174C	GATGTCAGCTCCTGCCGCAGCCGCACC
G207C	TGGCTCCGGGCCCAGTGTTATGCCAACCCCTTC
T240C	GAGCCAAAGTCCTGCCGGCTCTTCAC
E261C	GCTCCCGCCCCATGCAAGTCCAGGAAA
E292C	TTAACCCTTTATTGCCTAAGCACACCC
L329C	CTGCAGTACTCCTGCGCCGATGCCTGG
L357C	GCCACTATCACGCCTTGCATGTTCACAGGATAT
T386C	AAGCTGGTGAGAGAGTGCGAGCAGGGTGCTCTC
T417C	TCACTCTACCCAGCCTGTCTGAACTTTATGAAG