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. Author manuscript; available in PMC: 2011 May 21.
Published in final edited form as: J Mol Biol. 2010 Mar 31;398(5):657–671. doi: 10.1016/j.jmb.2010.03.048

Fig. 6. Mimicking Akt1-mediated phosphorylation of CtBP1 decreases dimerization.

Fig. 6

(a) COS1 cells were cotransfected with H6- or Flag-tagged CtBP1, CtBP(T176E) or CtBP(T176A) expression constructs, as indicated. Proteins were precipitated with anti-Flag agarose and analyzed by western blot with a H6 antibody. Expression of transfected proteins in the lysates is shown below. (b) COS1 cells were cotransfected with T7 tagged TGIF, Pc2, or MITR, together with a control vector, Flag-CtBP1 or CtBP(T176E) expression constructs. Proteins were precipitated with anti-Flag agarose and analyzed by western blot with a T7 antibody. Expression in the lysates is shown below.