Table 1.
Kinetic Analysis of Bacterial Growth. Optical Densities were measured at a Wavelength of 600 nm. Growth Rates (hr−1) were calculated using the Bacterial Growth Kinetic Software Package (F. Breidt, pers.comm). Values are reported as a mean (± sd). Bold numbers correspond to obtained values from high HMO consumers.
| Strain | ZMB1 without sugar | ZMB1 (2%Glucose) | Modified ZMB1 (2% HMO) | ΔOD | |
|---|---|---|---|---|---|
| Enterococcus faecalis OG1RF | Max. OD Growth rate |
0.370 (0.070) 0.053 (0.001) |
1.388 (0.054) 0.335 (0.001) |
0.245 (0.001) 0.039 (0.001) |
- |
| Enterococcus faecalis KA117 | Max. OD Growth rate |
0.403 (0.075) 0.079 (0.001) |
1.515 (0.017) 0.230 (0.001) |
0.309 (0.001) 0.027 (0.001) |
- |
| Streptococcus thermophilus MTC330 | Max. OD Growth rate |
0.388 (0.012) 0.028 (0.001) |
1.525 (0.188) 0.404 (0.001) |
0.469 (0.002) 0.026 (0.001) |
0.165 |
| Streptococcus thermophilus MTC360 | Max. OD Growth rate |
0.242 (0.082) 0.496 (0.026) |
1.329 (0.073) 0.330 (0.002) |
0.551 (0.001) 0.059 (0.001) |
0.243 |
| Bifidobacterium infantis ATCC15697 | Max. OD Growth rate |
0.175 (0.156) 0.015 (0.001) |
1.069 (0.062) 0.191 (0.001) |
0.698 (0.295) 0.023 (0.001) |
0.523 |
| Lactococcus lactis IL1403 | Max. OD Growth rate |
0.322 (0.008) 0.014 (0.001) |
1.353 (0.009) 0.253 (0.009) |
0.417 (0.001) 0.054 (0.001) |
0.124 |
| Eubacterium rectale ATCC35183 | Max. OD Growth rate |
0.305 (0.028) 0.047 (0.001) |
1.103 (0.028) 0.275 (0.002) |
0.454 (0.001) 0.052 (0.001) |
0.231 |
| Clostridium perfringens ATCC13124 | Max. OD Growth rate |
0.112 (0.007) 0.096 (0.031) |
0.937 (0.017) 0.147 (0.003) |
0.378 (0.001) 0.025 (0.001) |
0.177 |
| Clostridium perfringens AB1 | Max. OD Growth rate |
0.369 (0.055) 0.031 (0.001) |
1.471 (0.089) 0.117 (0.001) |
0.434 (0.001) 0.029 (0.001) |
0.171 |
| Clostridium difficile AB2 a | Max. OD Growth rate |
0.411 (0.078) 0,011 (0.001) |
1.108 (0.012) 0.184 (0.001) |
- - |
- - |
| Escherichia coli OP50 | Max. OD Growth rate |
0.288 (0.066) 0.073 (0.001) |
1.460 (0.220) 0.231 (0.001) |
0.386 (0.001) 0.056 (0.001) |
0.152 |
| Escherichia coli EC100 | Max. OD Growth rate |
0.319 (0.061) 0.053 (0.001) |
1.095 (0.149) 0.138 (0.001) |
0.383 (0.001) 0.042 (0.001) |
0.028 |
| Lactobacillus acidophilus NCFM | Max. OD Growth rate |
0.222 (0.029) 0.070 (0.001) |
0.815 (0.067) 0.010 (0.001) |
0.517 (0.001) 0.078 (0.001) |
0.290 |
| Bacteroides fragilis ATCC25285 | Max. OD Growth rate |
0.297 (0.001) 0.023 (0.001) |
1.352 (0.001) 0.068 (0.001) |
1.081 (0.001) 0.052 (0.001) |
0.940 |
| Bacteroides vulgatusATCC8482 | Max. OD Growth rate |
0.085 (0.002) 0.002 (0.001) |
1.049 (0.001) 0.629 (0.001) |
0.744 (0.001) 0.028 (0.001) |
0.660 |
| Veillonella parvula ATCC10790 | Max. OD Growth rate |
0.199 (0.001) 0.033 (0.001) |
1.220 (0.001) 0.081 (0.001) |
0.214 (0.001) 0.047 (0.001) |
0.015 |