Gene expression profile analysis of unknown 4766-1, unknown 4680-1, NADH, centaurin, and melanotransferrin mRNA during intestinal regeneration. Analysis was performed for all samples by conventional RT-PCR and normalized against cytochrome b (Cyt b, 161 bp). A: compared with normal animals, unknown 4766-1 presents a significant overexpression at 3dpe (*P < 0.01, 2-tailed t-test). Centaurin expression presents a significant increase in 7dpe (*P < 0.01) compared with 3dpe and normal animals. Melanotransferrin presents an increase tendency in 3dpe and 7dpe but is not statistically significant. Unknown 4680-1 and NADH expression didn't show a significant change. B: centaurin presents a significant overexpression in stages 7dpe–21dpe, where the peak of expression appears at 14dpe (**P < 0.001, 2-tailed t-test) compared with normal animals. Overexpression of melanotransferrin was detected in 3dpe+7dpe (*P < 0.05, 2-tailed t-test) compared with later stages of regeneration (21 +28dpe) and normal animals. Values are presented as means ± SE of 5 animals per group.