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ANP binding to ECD at varying halide concentrations. (a) Binding of 125I-ANP(4-28) to ECD was measured at varying concentrations of NaF (□), NaCl (•), NaBr (○), and NaI (Δ). Bars show the standard error in triplicate determinations. (b) Hill plots for chloride (•) and bromide (○) binding to ECD, in which the occupancy was calculated as the ANP-binding activity divided by the maximum activity at 100 mM halide concentration.
