Figure 2.

Two-dimensional NMR analysis of control workgroup proteins. Samples from the control workgroup were subject to ¹H-¹⁵N HSQC NMR. Spectra were acquired in ∼80 min using 16 transients per FID at 25°C on a Bruker Avance 600 MHz NMR equipped with a 5 mm TCI Cryoprobe. Purity of samples generated by the Maxwell-16 is illustrated by SDS-PAGE (inset). Each gel contains samples of depleted lysate (DL), each wash step (W1–W4) and pure protein in the elution cuvette (EC) prior to exchange into the selected NMR buffer.