Figure 3.

Two-dimensional NMR analysis of hPar-3 and β-PIX domain constructs. All 18 of the hPar-3 and β-PIX constructs were analyzed by ¹H-¹⁵N HSQC NMR. Spectra were acquired in ∼80 min using 16 transients per FID at 25°C on a Bruker Avance 600 MHz NMR equipped with a 5 mm TCI Cryoprobe. HSQC spectra for constructs that are folded, partially folded or unfolded are labeled in black and those that contain no signal are labeled in gray. The two proteins that are suitable for NMR structure determination are indicated with an asterisk.