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. Author manuscript; available in PMC: 2010 May 10.
Published in final edited form as: Nature. 2008 Jan 31;451(7178):587–590. doi: 10.1038/nature06515

Figure 3. DBC1 acts as an inhibitor of SIRT1 in human cells.

Figure 3

a, DBC1 represses SIRT1 deacetylation activity in vivo. Transfected H1299 cell extracts were analysed by western blot using antibodies as shown. b, DBC1 inhibits SIRT1-mediated co-immunoprecipitation of p53 in vivo. H1299 cells were transfected as indicated and Flag–SIRT1 immunoprecipitations were analysed by western blot as shown. c, DBC1 expression rescues SIRT1-mediated repression of p53 transcriptional activation. H1299 cells were transfected with the PUMA-luciferase promoter construct and p53, SIRT1 and DBC1 as indicated. Lysates were assayed for the dual-luciferase activity. Error bars represent s.d., n = 3. d, Model showing DBC1 acting as an inhibitor of SIRT1-mediated repression of p53.