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(a) Transmission and PM-IRRAS spectra for the amide region of C16 H31O - V4A4E3 (PA 4). (b) The hydrogen bond alignment quantified from the reduction of the Amide I absorbances (1630 cm−1) from transmission IR to PM-IRRAAS normalized to amibe II absorbance (1550 cm−) for PAs 1–6.