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. 2010 May 7;5(5):e10536. doi: 10.1371/journal.pone.0010536

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Non-transgenic (A) and transgenic N. benthamiana containing the RB gene (B) were infiltrated with A. tumefaciens containing the following constructs: 1) pGR106-INF1; 2) pGR106-GFP; 3) pGR106-ipiO4; 4) pGR106-ipiO1; 5) pGR106-INF1 + pGR106-ipiO4; 6) pGR106-INF1 + pGR106-GFP; 7) pGR106-ipiO1 + pGR106-ipiO4; 8) pGR106-ipiO1 + pGR106-GFP. Co-infiltration was accomplished using a mixture of equal amounts of Agrobacterium. The photograph, which is representative of multiple replications, was taken 7 days after agroinfiltration.