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. 2010 May 7;5(5):e10536. doi: 10.1371/journal.pone.0010536

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Transgenic N. benthamiana containing the RB gene was infiltrated with A. tumefaciens containing the following constructs: 1) pGR106-INF1; 2) pGR106-GFP; 3) pGR106-ipiO4; 4) pGR106-ipiO1; 5) pGR106-INF1 + pGR106-ipiO4; 6) pGR106-INF1 + pGR106-GFP; 7) pGR106-ipiO1 + pGR106-ipiO4; 8) pGR106-ipiO1 + pGR106-GFP. Co-infiltration was accomplished using a mixture of Agrobacterium strains. A contains 5-fold more and B contains 10-fold more Agrobacterium expressing INF1 or ipiO1 in 5, 6, 7, and 8 compared to the co-infiltrated construct. The photograph was taken 7 days after agroinfiltration.