Figure 5. Relationship between the sequence coverage for each clone and the reconstruction accuracy in terms of CDS structure consistency.

Clones in the simulated datasets were binned according to their per-clone sequence coverage. The bins were of every 10-fold. Note that every full-length cDNA clone was counted 10 times as it appeared with 10 different sequence coverage. We calculated the percentage of clones (left Y-axis) classified as consistent (or inconsistent) in terms of CDS structure for each bin. The number of clones in each bin is also shown (right Y-axis). Per-clone sequence coverage of 30-fold was sufficient to produce a CDS-consistent assembly in 95% of the cases, showing that uniform coverage distribution is highly desirable to achieve better efficiency.