Table 2. Assembly statistics and accuracy evaluation for human full-length cDNA clones.
Library 1 | Library 1+2 | ||
Reference full-length cDNA clones | (A) | 199 | 199 |
Reference clones amplified by PCR | (B) | 158 | 158 |
% | (B/A) | 79.4% | 79.4% |
Clones with CDS annotation | (C) | 141 | 139 |
% | (C/B) | 89.2% | 88.0% |
Clones with consistent CDS structure | (D) | 134 | 135 |
% | (D/C) | 95.0% | 97.1% |
Clones with inconsistent CDS structure | (E) | 7 | 4 |
% | (E/C) | 5.0% | 2.9% |
A: The number of the reference full-length cDNA clones used in the experiment. the original number was 200, but one of them was excluded due to chimerism; B: the number of reference full-length cDNA clones successfully amplified by PCR. Note that it does not include non-reference full-length cDNA clones; C: the number of successfully amplified reference full-length cDNA clones with CDS annotation. Non-reference and/or non-amplified full-length cDNA clones were excluded; D: the number of full-length cDNA clones that were reconstructed consistently in terms of CDS structure. See Fig. 2 for CDS structure consistency; E: the number of full-length cDNA clones that were not reconstructed consistently with regard to CDS structure. This always equals to C–D.