Table 1.

Titer (ng) of C₂₃ and C₂₅ pentaenes in various tissues of 1–2 day old virgin female navel orangeworm moths^a

Compound	Pheromone Gland		Abdomenb		Abdominal Cuticlec	Hemolymphd
	(N = 5)		(N = 5)		(N = 7)	(N = 2)
	photophase	scotophase	photophase	scotophase	scotophase	scotophase
3Z,6Z,9Z,12Z,15Z-23:H	0.40 ± 0.47	0.45 ± 0.47	135 ± 70	111 ± 39	17 ± 8	2.9 ± 0.2
3Z,6Z,9Z,12Z,15Z-25:H	0.03 ± 0.01	0.07 ± 0.05	10 ± 6	18 ± 11	2.0 ± 1.7	0.71 ± 0.12

^aIndividual 1- to 2-d-old intact virgin female moths (or in the case of hemolymph, samples of four females) were extracted during the last hour of photophase or the 6^th hour of scotophase, respectively. The titer of each polyene (mean ± SD) was determined by GC-MS in selected ion monitoring mode using the base peak (m/z 79) for quantification versus 3Z,6Z,9Z-21:H as internal standard. Student’s t-tests showed no significant differences at the 5% level between photophase and scotophase extracted polyenes in the pheromone gland or whole abdominal tissue

^bAbdomen = the whole abdomen with the pheromone gland removed, homogenized and extracted

^cAbdominal cuticle = the abdominal cuticle with the pheromone gland, hemolymph, fat body, and other tissues removed

^dHemolymph = the mixture of hemolymph and saline extracted from the abdomen, after injection of 10 µl Weever’s saline per female