Figure 1.

Astrocyte [Ca²⁺]_i transients evoked by activation of P2Y₁ and PAR-1 receptors. A, Representative images of the stratum radiatum region of the hippocampus with astrocytes showing increases in [Ca²⁺]_i during activation of PAR-1 receptors with TFLLR peptide (30 μm). Arrowheads point to astrocytes showing intracellular calcium transients. B, Exemplar traces PAR-1- and P2Y₁-evoked [Ca²⁺]_i transients. C, D, Pooled data for experiments such as those in A. The gray lines are individual cells, and the solid lines are the averages from all cells. Activation of both P2Y₁ and PAR-1 receptors evoked increases in astrocyte [Ca²⁺]_i levels. E, The average normalized traces for [Ca²⁺]_i transients evoked by P2Y₁ and PAR-1 receptor activation; note that there was a latency for the PAR-1-mediated responses in relation to those mediated by P2Y₁ receptors, and their duration was longer.