Figure 4.

Production of cyclase through high biomass cultivation. Expression was induced with methanol when the culture had reached a cell density of 200 g/L. After about 100 h of induction and production of about 180 mg/L of cyclase, 2.0 liter of the culture was harvested, the yeast cells were re-suspended in fresh medium and induction was continued with higher methanol levels for an additional 53 h. Reproduced with publisher’s permission from [56].