Table 3.
Continuous processes for marine enzyme production.
| Enzyme and source | Bioreactor scale and working volume | Process parameters | Carbon source(s) | Enzyme activity | Reference | |||
|---|---|---|---|---|---|---|---|---|
| pH | Temperature (°C) | Aeration rate (L/L/min) and agitation rate (rpm) | Time | |||||
| Chitinase (Paenibacillus sp. CHE-N1) | Biotop BTF-A5L (Taiwan) 5.0 L (reactor volume), 2.0 L (working volume) | 8.5 | 34.3 | 3, 200 | 132 h | Crab shell chitin powder 55.4 g/L | 42,800 mU/mL | [57] |
| α-glucosidase (Pyrococcus furiosus DSM 3638) | Anaerobic, 5.0 L (reactor volume), 2.0 L (working volume) | 6.8 | 98.0 | NR | NR | Yeast extract 0.1% | NR | [58] |
| L-glutaminase (Beauveria bassiana BTMF S-10) | Immobilized process; PBR, glass column 2.3 cm radius, 20.0 cm height | 9.0 | 27.0 | No aeration | 18 h | L-glutamine 0.25% w/v, D-glucose 0.5% w/v | 4.048 U/mL/h | [59] |
| L-glutaminase (Pseudomonas sp. BTMS-51) | Immobilized process; PBR, ID 3.6 cm, height 45.0 cm | 6.0 | 30.0 | NR | 120 h | L-glutamine 20 g/L, D-glucose 10 g/L | 13.49 U/mL/h | [60] |
| β-galactosidase (Pseudoalteromonas sp. 22b) | Immobilized process; PBR with substrate recycling | 7.6 | 15.0 and 4.0 | NR | 40 d | Lactose in milk | 3.7 U/mg protein | [61] |
NR: Not reported; PBR: Packed bed reactor; ID: Internal diameter.