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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Biomaterials. 2010 Mar 25;31(18):4987–4994. doi: 10.1016/j.biomaterials.2010.02.063

Figure 3. Release studies of proteins and encapsulated compounds from NTA-functionalized microparticles.

Figure 3

His6-GFP was tethered to the surface of the microparticle through the Ni-NTA interaction and release was measured under physiological conditions (pH 7.4, 37°C) using a fluorescence plate reader. Either Rhodamine-B or SB239063, a p38 inhibitor), was encapsulated in the core of the microparticle. (Note: SB239063 data published in Sy et al. [35] GFP was released from the microparticle with a half life of 2h with <95% release at 48h. Rhodamine-B showed a burst release with 38% released within the first 6h and 42% released after one week. SB239063 had a release half life of one week with 66% release at three weeks. (data shown: mean ± SEM, n=3)