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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Biomaterials. 2010 Mar 25;31(18):4987–4994. doi: 10.1016/j.biomaterials.2010.02.063

Figure 6. Microparticles can be targeted to HUVEC though VE-Cadherin.

Figure 6

(A) Fluorescence micrograph of HUVECs incubated with VE-Cadherin loaded microparticles. Microparticle cores were loaded with coumarin-6 for tracking. (scale bar 100 μm) (B) Representative fluorescence micrograph of HUVEC incubated with Ni-NTA microparticles. (scale bar 100 μm) (C) Laser scanning confocal micrograph of HUVEC with VE-Cadherin loaded microparticles. Orthogonal projection of z-stack suggests that microparticles are not internalized. (scale bar 20 μm) (D) Percentage of cells positive for at least one microparticle. Negative controls (HUVEC+Ni-NTA, NCM+VE-Cadherin, NCM+Ni-NTA) ranged from 19-26% of cells positive for one microparticle. By contrast, HUVECs incubated with VE-Cadherin loaded microparticles had 45.7% of cells labeled by microparticles, which represents a 2.4-fold increase compared to HUVECs treated with non-targeted microparticles. (data shown: mean ± SEM)