Figure 4. CycC-CTAP protein levels in response H₂O₂ treatment.

Ax2[cycC::cycC-CTAP] and Ax2[act15::FLAG-cycC] cells were harvested, washed and shaken in KK₂ buffer at 1×10⁷ cells/ml. After 1 hr the cells were split and incubated in the presence (+) or absence (−) of 0.25 mM H₂O₂. Western samples were taken after 1, 2 and 3 hrs of treatment and analysed on a 10% SDS-PAGE gel, transferred to a nitrocellulose membrane and probed with an α-mouse IgG secondary antibody and an α-actin antibody to control for loading.