Figure 6. Analysis of myc-Cdk8 complexes in the presence and absence of FLAG-CycC.
A. Nuclear extracts from 1×109 Ax2[myc-cdk8, FLAG-cycC] or Ax2[myc-cdk8] cells (annotated as FLAG-CycC + and - respectively) were fractionated by gel filtration on a Superose 6 column and analysed as described in the legend to Figure 5. B and C. Samples were harvested from vegetatively growing cells and analysed by western (B and C) and northern (C) blot. Western blots samples were analysed and probed with the α-FLAG antibody to recognise FLAG-CycC and against the myc epitope to recognise myc-Cdk8. RNA samples were resolved on a 1% formaldehyde gel, transferred to a nylon membrane and probed with a 32P labelled fragment of the cycC gene. The blot was reprobed with a 32P labelled fragment of the IG7 gene to control for loading.