Figure 1. 9mer flu-derived peptides induce CD4⁺ or CD8⁺ T cell responses.

Peripheral blood mononuclear cells (PBMC) obtained from donors 21 (A), 25 (B), 29 (C) 23 (D), 35 (E), 32 (F) and 21 (G) were depleted of CD4⁺ T cells or CD8⁺ T cells and incubated with the indicated peptides for 10 days. Prior to testing, cells were harvested, washed and incubated in enzyme-linked immunospot (ELISPOT) plates for 20 h in the absence or presence of the indicated peptides. Results are expressed as the mean spot-forming cell values of four replicate ELISPOT microcultures, each containing 1×10⁵ CD4⁺ or CD8⁺ T cell-depleted PBMC. *, P<0.05 (Student's t- test).