Table 6. Affinities of peptides to MHC-II alleles.
| Peptide sequence | DRB1*0101 | DRB1*0301 | DRB1*0302 | DRB1*0401 | DRB3*0301 | DRB5*0101 | DPA1*0103/DPB1*0401 | |
| HA306–318 | YKYVKQNTLKLAT | 108 a | 26 | 53 | 15 | 3 | 12 | NB |
| hypothetical protein 239–253 [27] | YILLKKILSSRFNQM | 435 | 20 | 156 | 107 | 3 | 17 | 25 |
| DR/Donor 1 b | IMLKANFSV (PF-103) | 3433 c | NB | NB | 1387 | 18 | NB | NB c |
| DR/Donor 25 | ETIVLLRAF (PF-113) | 3729 | NB c | NB | NB | NB | 780 c | NB |
| DP/Donor 23 | LVSLGAISF (PF-148) | 4340 c | NBc | NB | NB | NB | NB | NB c |
| DR/Donor 32 | YVKQNTLKL (PF-152) | 1203 | NB | 2221 | 226 c | 356 | 405 | NB c |
| DP/Donor 23 | IAPWYAFAL (PF-154) | NB c | NB c | NB | NB | NB | 1696 | NB c |
| Donor 17 d | LMNELGVPF (PF-141) | NB | NB | NB | NB | 4014 | NB | NB |
| DP/Donor 23 | LPFHNVHPL (PF-147) | NB c | NB c | NB | NB | NB | 3704 | NB c |
| DP/Donor 31 | LPFQNVHPV (PF-150) | NB | NB | NB | NB | NB | 1204 c | NB c |
Note: peptides marked in bold are positive controls included in the assay; HA306–318 is a promiscuous HLA-DR binder whereas hypothetical protein 239–253 has been shown to bind most HLA-DR alleles and the HLA-DP allele. Peptide showing affinities above 5000 nM are categorized as non-binders (NB).
a
Peptide binding affinity (KD) in nM.
b
Peptide reactivity blocked by anti-HLA-II subtype antibody in the responding donor number (See Fig. 2 and Table 5).
c
The HLA-II subtype assayed for peptide binding was expressed by donor.
d
No cells available for HLA-II typing.