Figure 2. The C-terminus of NCS-1 is particularly affected by the R102Q mutation.

(A) Distribution of resonances along the NCS-1 sequence that are the shifted or undetectable in the NCS-1(R102Q) ¹H/¹⁵N HSQC spectrum. (B) Structure of the C-terminal half of NCS-1 (residues 93–186 rendered from the PDB file1G8I) showing the position of R102 within helix F and the hydrogen bond interactions between helix F and the C-terminal domain. (C) Coomassie blue-stained SDS-polyacrylamide gel showing the NCS-1 and NCS-1(R102Q) proteins after analysis by NMR spectroscopy.