Fig. 1.

Detection and measurement of AEA by LC/MS in L. variegatus embryos. Ion chromatograms of AEA and AEA-d₈ in A 8–16 cell stage and B mid-blastula 2 stage embryos. AEA was quantified using AEA-d₈ as internal standard, where 90 pmol was added to the sea urchin samples. The amounts of AEA measured at the 8–16 cell and mid-blastula stage were 1.33 ± 0.12 and 6.8 ± 3.5 pmol/g, respectively.