Fig. 2.

Dose-response effects of AEA on L. variegatus sea urchin embryos. AEA was introduced at the 2-cell stage (2 h post-fertilization) and larvae imaged at the pluteus stage. A Untreated control; B vehicle control; C AEA 5 μM; D AEA 10 μM; E AEA 20 μM; F AEA 25 μM. AEA dose-dependently perturbed development of embryos/larvae compared with controls, preventing them from developing the typical morphology of pluteus larvae, with extended ‘arms’ (arrows). At the highest doses E, F cells accumulated in the blastocoel and the ectodermal wall was thickened (F inset, arrowhead). Scale bar = 100 μM A–C; 50 μM D–F. Asterisk = Cells in blastocoel.