Fig. 1.

Increased abundance of dSesn upon TOR activation. Larval wing discs of indicated strains were stained to visualize indicated proteins or mRNA. The dorsal side points upwards. Dorsoventral boundary (D/V in A) was visualized by staining with an antibody to the wingless (Wg) protein (red). (A to C) Expression of dSesn protein (green) in the absence (A) or presence of InR^CA in WT (B) and dSesn-null (C) strains. (D and E) Accumulation of dSesn mRNA (green) in response to InR^CA detected by in situ hybridization. (F) The signaling network controlling TOR activity and expression of dSesn. (G to K) Accumulation of dSesn (green) in response to Rheb (G) but not S6K^CA (J) or loss of 4E-BP (K). Thor¹ is a Drosophila 4E-BP loss-of-function mutant. (H and I) Accumulation of dSesn after somatic loss of PTEN (H) or TSC1 (I). Absence of GFP (green) indicates loss of PTEN or TSC1 resulting in dSesn (red) accumulation.