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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cell Calcium. 2009 Jun 11;46(1):73–84. doi: 10.1016/j.ceca.2009.05.003

Fig. 1.

Fig. 1

TRPC4-reporter mRNA is suppressed by TRPC4-shRNA constructs in AD-293, PHM1-41 and UtSMC cells. (A) Significant suppression of luminescence from a Renilla luciferase-TRPC4 fusion reporter (psiTC4) following co-transfection of the reporter and plasmids expressing TRPC4 shRNAs (TC4sh1-4) in AD-293 cells as described in Methods. Controls include empty vector, puc-19 vector and scrambled shRNA (shx1). (B) psiTC4 was electroporated into PHM1-41 and UtSMC cells, together with TRPC4-shRNA#1 (TC4sh1) in pSHAG vector as described in Methods. Controls include empty vector (pSHAG) and pSHAG plasmid expressing a scrambled shRNA control sequence (shx1). In A and B samples were isolated >72 h post-electroporation. Data are expressed as % of luminescence exhibited by co-transfection of empty vector and represent mean ± S.E.M. (n=3). Background luminescence (∼7%) was subtracted from all samples. Data were analyzed by one-way ANOVA and Tukey's test. Significant difference between groups (p<0.05) is indicated by different lowercase letters.